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CL1 BSO SOP GLP: Difference between revisions
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The typical stain used is Ethidium Bromide which is a fluorophore that is excited within the UV spectrum (with excitation maxima under 300nm) and emission within the visible spectrum. This presents two main risks to the user: | The typical stain used is Ethidium Bromide which is a fluorophore that is excited within the UV spectrum (with excitation maxima under 300nm) and emission within the visible spectrum. This presents two main risks to the user: | ||
i) As a DNA intercalating agent Ethidium Bromide is potentially mutagenic and should never be allowed to come in contact with the user’s bare skin, this applies through the whole DNA electrophoresis procedure from gel preparation, through electrophoresis and then subsequent gel visualisation. This is therefore also carried out in the electrophoresis/ethidium bromide area of the laboratory bench. | i) As a DNA intercalating agent Ethidium Bromide is potentially mutagenic and should never be allowed to come in contact with the user’s bare skin, this applies through the whole DNA electrophoresis procedure from gel preparation, through electrophoresis and then subsequent gel visualisation. This is therefore also carried out in the electrophoresis/ethidium bromide area of the laboratory bench. | ||
ii) UV light can be damaging to exposed surfaces of the body and in extreme circumstances or prolonged use lead to carcinomas or eyesight damage. It is therefore advisable that eye protection be used by all people present within the laboratory when UV gel visualisation is taking place and that any possible contact from UV radiation on the user’s body while manipulating and viewing their be minimised through adequate PPE and controlled use of the UV light source. | ii) UV light can be damaging to exposed surfaces of the body and in extreme circumstances or prolonged use lead to carcinomas or eyesight damage. It is therefore advisable that eye protection be used by all people present within the laboratory when UV gel visualisation is taking place and that any possible contact from UV radiation on the user’s body while manipulating and viewing their gel be minimised through adequate PPE and controlled use of the UV light source. | ||
*** UV GEL BOX NEEDS FIXING BEFORE AUDIT!!!!******* | *** UV GEL BOX NEEDS FIXING BEFORE AUDIT!!!!******* | ||
