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LBL04002: Difference between revisions
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The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures generated, steam and high pressures. | The operator of the autoclave is responsible for their own safety and that of others in the vicinity during its use. The particular risks presented in using this piece of equipment are primarily due to the high temperatures generated, steam and high pressures. | ||
The operator is responsible for ensuring complete sterilization of containers, glassware, tubes, media and equipment. To ensure environmental microbes do not contaminate the target microbe strain. | |||
'''4. Materials''' | '''4. Materials''' | ||
... | The main consumables for the autoclave are RO water and autoclave tape. | ||
RO water needs to be regularly topped up, into the hatch on the top of the machine. | |||
The autoclave tape should be used to seal items to be sterilized and is stored on the shelf nearby. | |||
'''5. Related documents''' | '''5. Related documents''' | ||
... | http://www.phytotechlab.com/pdf/SterilizingNutrientMedia.pdf | ||
'''6. Definitions''' | '''6. Definitions''' | ||
None given | |||
