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Project:OD600 Measurement: Difference between revisions
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In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. The measured OD600 gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. 600nm is in the visible wavelength an is approximately orangey-red. | |||
The procedure is normally carried out using a visible light capable spectrophotometer like this one; | |||
[[File:15087049272 feb8d357df c.jpg|300px]] | |||
The idea is to take a 600 nm source, (or a source including 600 nm and a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer.. | The idea is to take a 600 nm source, (or a source including 600 nm and a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer.. | ||
