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LBL02001: Difference between revisions
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Add a couple of SOP refs and TODOs
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(Add a couple of SOP refs and TODOs) |
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**TODO**: SOP for kill bin required? If so add refs in sink induction section | |||
**TODO**: SOP for "storage and recovery of frozen bacterial stocks and procedures" required -- ref from section 6.15 | |||
'''1. Purpose''' | '''1. Purpose''' | ||
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'''6.10 Autoclave''' | '''6.10 Autoclave''' | ||
The autoclave is used to destroy any potential microorganisms that might contaminate media, reagents and consumables to be used aseptically in micro- and molecular biological procedures. High temperature and pressure is used to kill contaminants. The high temperature and pressure is accompanied by a vacuum cycle. Users should familiarise themselves with the instructions for use [REF] before working with the autoclave. The main risks to the user are heat of the metal pressurised unit during sterilisation and any vented steam. It is recommended that the autoclave and contents are allowed to cool for a while before handling. The correct procedures for using the autoclave/steriliser to prepare media and destroy GM waste are described in the SOPs. | The autoclave is used to destroy any potential microorganisms that might contaminate media, reagents and consumables to be used aseptically in micro- and molecular biological procedures. High temperature and pressure is used to kill contaminants. The high temperature and pressure is accompanied by a vacuum cycle. Users should familiarise themselves with the instructions for use [REF] before working with the autoclave. The main risks to the user are heat of the metal pressurised unit during sterilisation and any vented steam. It is recommended that the autoclave and contents are allowed to cool for a while before handling. The correct procedures for using the autoclave/steriliser to prepare media and destroy GM waste are described in the SOPs LBL04002 (Using the Autoclave for Media Preparation) and LBL04006 (Using the Autoclave for Waste Deactivation). | ||
'''6.11 Sink area''' | '''6.11 Sink area''' | ||
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The sink area should remain clear. All glassware should be kept clean and out of the way. | The sink area should remain clear. All glassware should be kept clean and out of the way. | ||
The sink is connected to the municipal drain. No biological material should be disposed of in the sink unless it has been inactivated appropriately (see LBL04006 for autoclave-based inactivation). Dangerous reagents and chemicals, in particular Ethidium Bromide, should not be poured down the sink. If there is any doubt about whether a particular chemical or reagent can be disposed of safely, consult the BSO. | |||
'''6.12 Chemicals and Storage''' | '''6.12 Chemicals and Storage''' | ||
