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LBL02001: Difference between revisions
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SOP refs and TODOs part 2
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(SOP refs and TODOs part 2) |
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**TODO**: SOP for "storage and recovery of frozen bacterial stocks and procedures" required -- ref from section 6.15 | **TODO**: SOP for "storage and recovery of frozen bacterial stocks and procedures" required -- ref from section 6.15 | ||
**TODO** SOP for gel visualisation (search 'gel vis') | |||
**TODO**: List of MSDSes (search page) | |||
**TODO**: List of chemicals (search page) | |||
**TODO**: PCR docs link for 6.5 PCR Machine | |||
**TODO**: SOPs for microwave (6.3) | |||
'''1. Purpose''' | '''1. Purpose''' | ||
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'''TODO''' It's not clear whether the inductee should notify the Committees or the BSO prior to commencing work. It seems like the inductee should notify the BSO, and it is the BSO's responsibility to make sure the cttees are aware. Either way, clarification required. | '''TODO''' It's not clear whether the inductee should notify the Committees or the BSO prior to commencing work. It seems like the inductee should notify the BSO, and it is the BSO's responsibility to make sure the cttees are aware. Either way, clarification required. | ||
For those starting new projects in the laboratory, full details of the requirements of principal investigators, in particular risk assessments for new projects, can be found in | For those starting new projects in the laboratory, full details of the requirements of principal investigators, in particular risk assessments for new projects, can be found in LBL06001, Project Risk Assessment Procedure for Lab Users Proposing New Projects. | ||
'''6.2 PPE, Biosafety and Contamination Risks''' | '''6.2 PPE, Biosafety and Contamination Risks''' | ||
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'''6.4 Spectrophotometer''' | '''6.4 Spectrophotometer''' | ||
The spectrophotometer is a device that measure the absorbance of a given liquid sample at various wavelengths of light. A full description of its usage is given in the | The spectrophotometer is a device that measure the absorbance of a given liquid sample at various wavelengths of light. A full description of its usage is given in LBL04001, Operating the Spectrophotometer. Users should be aware that this system can generate light of harmful intensities in both ultraviolet and infrared wavelengths. While most of the optical path and the bulbs required to generate this light is contained within the system, it is beamed into the sample measurement compartment of the device. As such the spectrophotometer should be operated only with the sample measurement compartment fully closed. | ||
'''6.5 PCR Machine''' | '''6.5 PCR Machine''' | ||
The Polymerase Chain Reaction (PCR) machine, also known as a thermal cycler, is used to amplify specific sections of template DNA either for diagnostic or molecular cloning purposes. The heating block and underside lid of this machine will typically reach temperatures in the region of 95 to 100degC in a typical amplification program and caution should be exercised when placing and removing samples. Information regarding the current state of the machine including current program and temperature is displayed on the LCD on the front panel of this machine. Full information regarding the use and programming of the Techne thermal cycler and its use in various procedures can be found in the | The Polymerase Chain Reaction (PCR) machine, also known as a thermal cycler, is used to amplify specific sections of template DNA either for diagnostic or molecular cloning purposes. The heating block and underside lid of this machine will typically reach temperatures in the region of 95 to 100degC in a typical amplification program and caution should be exercised when placing and removing samples. Information regarding the current state of the machine including current program and temperature is displayed on the LCD on the front panel of this machine. Full information regarding the use and programming of the Techne thermal cycler and its use in various procedures can be found in LBL07004, Setting up and running PCRs, and the technical documentation. [docs] | ||
'''6.6 Electrophoresis Power Supply Unit''' | '''6.6 Electrophoresis Power Supply Unit''' | ||
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'''6.9 HEPA flow cabinet/area''' | '''6.9 HEPA flow cabinet/area''' | ||
The HEPA-filtered laminar flow unit allows us to work in sterile air in order to prevent contamination of our work, such as petri dishes and broths, with other microorganisms. Proper usage of the laminar flow area is described in the SOPs. It should be noted that the laminar flow functions in such a way as to protect the user’s work rather than the user, and so caution must still be exercised by the user when maintain the aseptic conditions of materials used within the flow area, and in disposal of consumables to assist in containment of biomaterial. For more information on aseptic technique, see the | The HEPA-filtered laminar flow unit allows us to work in sterile air in order to prevent contamination of our work, such as petri dishes and broths, with other microorganisms. Proper usage of the laminar flow area is described in the SOPs. It should be noted that the laminar flow functions in such a way as to protect the user’s work rather than the user, and so caution must still be exercised by the user when maintain the aseptic conditions of materials used within the flow area, and in disposal of consumables to assist in containment of biomaterial. For more information on aseptic technique, see LBL04005, Operating the Laminar Flow Unit for asceptic sample handling. | ||
'''6.10 Autoclave''' | '''6.10 Autoclave''' | ||
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'''6.13 Incubator''' | '''6.13 Incubator''' | ||
The incubator is typically used for the culture of mesophiles, and, as such, does not operate at dangerous temperatures. Care should be taken when moving samples to and from the incubator as per handling biocontaminants mentioned previously. Many procedures described later in the SOP documentation indicate the need for incubation | The incubator is typically used for the culture of mesophiles, and, as such, does not operate at dangerous temperatures. Care should be taken when moving samples to and from the incubator as per handling biocontaminants mentioned previously. Many procedures described later in the SOP documentation indicate the need for incubation. Please refer to LBL04004, Using The Incubator, for more information. | ||
'''6.14 Centrifuges''' | '''6.14 Centrifuges''' | ||
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There are also two blood centrifuges which spin in the region of 1000 to 2000 rpm. These centrifuges do not have safety interlocks, so it is possible to start these centrifuges with their lids open. This is highly dangerous, as there is a risk both of exposure to machinery moving at dangerous speeds, and of spray from poorly-sealed liquid samples. Users of these centrifuges must ensure that the lids are closed prior to operation. | There are also two blood centrifuges which spin in the region of 1000 to 2000 rpm. These centrifuges do not have safety interlocks, so it is possible to start these centrifuges with their lids open. This is highly dangerous, as there is a risk both of exposure to machinery moving at dangerous speeds, and of spray from poorly-sealed liquid samples. Users of these centrifuges must ensure that the lids are closed prior to operation. | ||
All centrifuges should be cleaned after use for the purpose of biosafety, containment and equipment maintenance. | All centrifuges should be cleaned after use for the purpose of biosafety, containment and equipment maintenance. | ||
For more information on the use of the centrifuges, refer to | For more information on the use of the centrifuges, refer to LBL04003, The BioLab Centrifuges and their usage. | ||
