LBL04002: Difference between revisions

LBL04002 (view source)

569 bytes added , 11 December 2014

m

761

edits

@@ Line 53: / Line 53: @@
 .1 Sterilization of liquid media
-.1.1 Use the  * Sterilize the load using the Quick-Programme (15 psi/121 C/15 mins)
+.1.1 For sterilization of liquid media up to 1 litre, that requires 15 minutes @ 15 psi and 121 C, please use the "Gentle Programme";
+[[File:Screenshot-gentle-programme.png]]
+.1.2 For sterilization of liquid media of greater volumes than 1 litre, you need to preheat the autoclave and place the material to be sterilized in the autoclave 15 minutes before starting the programme. This is to ensure that the bulk volume of liquid comes up to temperature in time for the main sterilization phase.
+[[File:Screenshot-preheat.png]]
+.1.3 Then run for 15 minutes @ 15 psi and 121 C using the "Gentle Programme";
+[[File:Screenshot-gentle-programme.png]]