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LBL04006: Difference between revisions
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→Using the Autoclave for Waste Deactivation
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'''1. Purpose''' | '''1. Purpose''' | ||
This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures:<br> | This describes the operation of the Melag EuroKlav 29V-S. The autoclave is used for two main procedures with respect to GMO deactivation:<br> | ||
i) sterilization of | i) sterilization of glassware and equipment for reuse | ||
ii) Deactivation of | ii) Deactivation of small volumes of GMO exposed media, and GMO exposed waste and equipment. | ||
iii) | |||
'''2. Scope''' | '''2. Scope''' | ||
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7.1 Daily Test procedure | 7.1 Daily Test procedure | ||
Before using the autoclave for GMO deactivation, the operator should run a test strip through the autoclave to check that it is working to specification. | Before using the autoclave for GMO deactivation each day, the operator should run a test strip through the autoclave to check that it is working to specification. | ||
The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations. | The test strip should be stapled into the lab book, with a note indicating the operator and the date/time the test was run, along with any observations. | ||
7.1.1 | 7.1.1 Unwrapped equipment and glassware with no internal cavities can be sterilized using the Quick Programme (28 psi/134 C/3 mins) | ||
[[File:Screenshot-quick-programme.png]] | |||
7.1.2 Materials such as a solid and liquid media can be sterilized using the Universal Programme (28 psi/134 C/30 mins) | |||
[[File:Screenshot-universal-programme.png]] | |||
7.1.3 Large volumes of liquid or solid media should be processed with extra care. | |||
The autoclave should be preheated; | |||
[[File:Screenshot-preheat.png]] | |||
If possible, the media should be split into several autoclaves sessions. The load should be run under the Universal programme | |||
[[File:Screenshot-universal-programme.png]] | |||
