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Project:OD600 Measurement: Difference between revisions
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In the molecular biology of bacteria, OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 of the sample relative to a plain reference sample, gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. | In the molecular biology of bacteria, it is often useful to obtain bacteria at the most productive stage of their population growth cycle. This stage is known as exponential phase, and more specifically the mid-exponential phase of growth is the most productive of that. This phase is also referred to as the "log phase", because plotting the log of the cell number against time produces a straight line. [[File:Bacterial growth.png | ||
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OD600 is often used to estimate the current growth phase of a bacteria or other cells in suspension. It means the "optical density of the sample to light of 600nm". The measured OD600 of the sample relative to a plain reference sample, gives an estimate of the concentration of the cells in the liquid, which can be used to estimate the growth phase of the population. | |||
The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | The basic idea is to take a sample of plain media, and a sample of media in which bacteria has been growing. Initially the levels of bacteria will be very low, and the amount of light absorbed by the 2 samples will be similar. | ||
