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Project:OD600 Measurement: Difference between revisions
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[[File:Bacterial growth.png|thumb|right]] | [[File:Bacterial growth.png|thumb|right]] | ||
In the molecular biology of bacteria, it is often useful to obtain bacteria at the most productive stage of their population growth cycle. This stage is known as exponential phase, and more specifically the mid-exponential phase of growth is the most productive of that. This phase is also referred to as the "log phase", because plotting the log of the cell number against time produces a straight line. So a protocol might say something like "take a mid-log phase culture of DH5-a...." | In the molecular biology of bacteria, it is often useful to obtain bacteria at the most productive stage of their population growth cycle. This stage is known as exponential phase, and more specifically the mid-exponential phase of growth is the most productive of that. This phase is also referred to as the "log phase", because plotting the log of the cell number against time produces a straight line. So a protocol might say something like "take a mid-log phase culture of DH5-a...." and what it is saying is to start a culture of bacteria, and let it grow until it is mod-log-phase, and then use them at that point in time. | ||
== OD600 ??? == | == OD600 ??? == | ||
