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Project:OD600 Measurement: Difference between revisions
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== OD600 device == | == OD600 device == | ||
The idea is to make a cheap, robust, reliable alternative to a dedicated and expensive device. The initial brain storming is to take a 600 nm source, (or a broad wavelength source of light which includes 600 nm and use a BP filter) and a level detector, and calibrate it to match 0.4 at OD600 on a proper spectrophotometer.. | |||
[[File:OD600-proto-1.png|450px]] | |||
The initial attempt was to use a white led with a filter for something approximating 600nm | |||
[[File:14853907550 b7212c3efe k.jpg|350px]] | |||
At the moment the main issue is that the LDR (light dependent resistor) only sees about 20 ohms of difference between the initial inoculation and the mid-log phase, which shows as 0.4 OD600 on a spectrophotometer. | |||
It really needs some amplification and a lot more light shielding. | |||
the second idea (once the 605nm LEDs had turned up from ebay); | |||
[[File:OD600-proto-2.png|450px]] | |||
<gallery> | <gallery> | ||
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</gallery> | </gallery> | ||
If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample | If you don't have access to a spectrophotometer, (or even if you do), this should be a convenient way to estimate OD600 values for your sample | ||
