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LBA: Difference between revisions
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* You want 100ug/ml, meaning 10mg in 100ml | * You want 100ug/ml ultimately [http://openwetware.org/wiki/Ampicillin for high copy plasmids], meaning 10mg in 100ml | ||
* So you can use ampicillin stock solution (1000x conc) at 1ul / ml. | * So you can use ampicillin stock solution (1000x conc) at 1ul / ml. | ||
==Make new stock== | ==Make new stock== | ||
* Needs to be 1000x, so 100mg per ml | |||
Solubility rating is 50mg/ml, so make 1ml of stock. | Solubility rating is 50mg/ml, so make 1ml of stock. | ||
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Microwave? | Microwave? | ||
Add ampicillin at 48C | |||
