Biohacking Wishlist: Difference between revisions
No edit summary |
mNo edit summary |
||
| (15 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
== | == Lab Balance == | ||
We | We have several pieces of equipment that need calibrating. | ||
== PCR machine == | |||
We currently have 1 working PCR machine, but it's quite old. | |||
== Lab balance with RS-232/usb outputs == | |||
http://www. | It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better; | ||
http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html | |||
http:// | or possibly a UK supplier of something like this; | ||
http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm | |||
== Visible Spectrophotometer for OD600 == | |||
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison. | |||
Something like this would probably do; | |||
http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg | |||
== UV Spectrophotometer == | |||
this would also be useful, but unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP; | |||
https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb | |||
In order to improve the general hygiene | == Nanodrop spectrophotometer == | ||
"Microvolume UV-Vis spectrophotometers and fluorospectrometers" | |||
http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf | |||
== Ultra Low Temp Freezer (-80C, -70C, or comparable) == | |||
For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard. | |||
Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells. | |||
==<s>Foot pedal operated lab waste bin</s>== | |||
(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!) | |||
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves. | |||
<gallery> | <gallery> | ||
| Line 33: | Line 48: | ||
</gallery> | </gallery> | ||
==Lab consumables== | |||
large gloves | |||
disposable pipettes | |||
Sterile petri dishes | |||
Gas can for bunsen burner | |||
== Agarose == | == Agarose == | ||
For making gels and stuff. It's also not that cheap... | |||
<gallery> | <gallery> | ||
| Line 57: | Line 72: | ||
http://www.melford.co.uk/index.php?t=details&sid=\&&id=104 | http://www.melford.co.uk/index.php?t=details&sid=\&&id=104 | ||
Latest revision as of 20:24, 13 November 2015
Lab Balance
We have several pieces of equipment that need calibrating.
PCR machine
We currently have 1 working PCR machine, but it's quite old.
Lab balance with RS-232/usb outputs
It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better; http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html
or possibly a UK supplier of something like this; http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm
Visible Spectrophotometer for OD600
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison. Something like this would probably do; http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg
UV Spectrophotometer
this would also be useful, but unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP; https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb
Nanodrop spectrophotometer
"Microvolume UV-Vis spectrophotometers and fluorospectrometers" http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf
Ultra Low Temp Freezer (-80C, -70C, or comparable)
For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.
Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.
Foot pedal operated lab waste bin
(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!)
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.
Lab consumables
large gloves
disposable pipettes
Sterile petri dishes
Gas can for bunsen burner
Agarose
For making gels and stuff. It's also not that cheap...
SKU: NBS-AG500 £171.00
http://www.nbsbio.co.uk/nbs-ag500
500g 149.00 €
