Biohacking Wishlist: Difference between revisions

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== Service manual for a Unicam Helios Beta Spectrophotometer ==
== Lab Balance ==


We received a donated [https://wiki.london.hackspace.org.uk/w/index.php?title=Spectrophotometer spectrophotometer] which is currently not working. would like to do a repair and the service manual would be helpful;
We have several pieces of equipment that need calibrating.


The part number for the Helios Alpha, Beta, Gamma and UV1 Service Manual is 9499 235 06011
== PCR machine ==


https://farm3.staticflickr.com/2936/14794946891_a616cdbabe_c.jpg
We currently have 1 working PCR machine, but it's quite old.


==Foot pedal operated lab waste bin==
== Lab balance with RS-232/usb outputs ==


In order to improve the general hygiene and to minimise the risk of loss of containment of GMO, it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal)
It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better;
http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html
 
or possibly a UK supplier of something like this;
http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm
 
== Visible Spectrophotometer for OD600 ==
 
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison.
Something like this would probably do;
http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg
 
== UV Spectrophotometer ==
 
this would also be useful, but unfortunately replacement deuterium bulbs are expensive.  seem like at least 100GBP;
https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb
 
 
 
== Nanodrop spectrophotometer ==
 
"Microvolume UV-Vis spectrophotometers and fluorospectrometers"
http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf
 
== Ultra Low Temp Freezer (-80C, -70C, or comparable) ==
 
For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.
 
Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.
 
==<s>Foot pedal operated lab waste bin</s>==
(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!)
 
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.


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New they are quite expensive, but I am sure we can find one on ebay, or to repair etc;
==Lab consumables==


http://www.medisave.co.uk/clinical-waste-hands-free-bin-50-litre-yellow-lid-p-7466.html
large gloves


http://www.wybone.co.uk/clinical-waste-bins/sackholders/
disposable pipettes


http://officebins.co.uk/pedal-medical-bins/open-body-hands-free-no-wheels-20-litre
Sterile petri dishes


http://www.nisbets.co.uk/4/Cleaning-and-Hygiene-Waste-Management-Bins-Sack-Trolley/c03c02c01c02.r10.1
Gas can for bunsen burner


== Agarose ==
== Agarose ==


molecular biologists get through this stuff like there is no tomorrow. It's for making gels and stuff. It's also not that cheap...
For making gels and stuff. It's also not that cheap...


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http://www.melford.co.uk/index.php?t=details&sid=\&&id=104
http://www.melford.co.uk/index.php?t=details&sid=\&&id=104
==Lab consumables==
large gloves
disposable pipettes
Sterile petri dishes
Gas can for bunsen burner

Latest revision as of 20:24, 13 November 2015

Lab Balance

We have several pieces of equipment that need calibrating.

PCR machine

We currently have 1 working PCR machine, but it's quite old.

Lab balance with RS-232/usb outputs

It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better; http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html

or possibly a UK supplier of something like this; http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm

Visible Spectrophotometer for OD600

A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison. Something like this would probably do; http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg

UV Spectrophotometer

this would also be useful, but unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP; https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb


Nanodrop spectrophotometer

"Microvolume UV-Vis spectrophotometers and fluorospectrometers" http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf

Ultra Low Temp Freezer (-80C, -70C, or comparable)

For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.

Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.

Foot pedal operated lab waste bin

(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!)

In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.

Lab consumables

large gloves

disposable pipettes

Sterile petri dishes

Gas can for bunsen burner

Agarose

For making gels and stuff. It's also not that cheap...

SKU: NBS-AG500 £171.00

http://www.nbsbio.co.uk/nbs-ag500

500g 149.00 €

http://www.melford.co.uk/index.php?t=details&sid=\&&id=104