Biohacking Wishlist: Difference between revisions

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== Coomassie brilliant blue  ==
We currently need "Coomassie Brilliant Blue 1" to be able to perform Bradform protein assays
"The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia
*  Lyophilized bovine plasma gamma globulin
*    Coomassie Brilliant Blue 1
*    0.15 M NaCl
*    Spectrophotometer and tubes
*    Micropipettes
http://www.nbsbio.co.uk/cb0037-25g
http://en.wikipedia.org/wiki/Coomassie_Brilliant_Blue
== Service manual for a Unicam Helios Beta Spectrophotometer ==
== Service manual for a Unicam Helios Beta Spectrophotometer ==



Revision as of 16:24, 22 August 2014

Coomassie brilliant blue

We currently need "Coomassie Brilliant Blue 1" to be able to perform Bradform protein assays

"The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia


  • Lyophilized bovine plasma gamma globulin
  • Coomassie Brilliant Blue 1
  • 0.15 M NaCl
  • Spectrophotometer and tubes
  • Micropipettes

http://www.nbsbio.co.uk/cb0037-25g

http://en.wikipedia.org/wiki/Coomassie_Brilliant_Blue


Service manual for a Unicam Helios Beta Spectrophotometer

We received a donated spectrophotometer which is currently not working. would like to do a repair and the service manual would be helpful;

The part number for the Helios Alpha, Beta, Gamma and UV1 Service Manual is 9499 235 06011

14794946891_a616cdbabe_c.jpg

Foot pedal operated lab waste bin

In order to improve the general hygiene and to minimise the risk of loss of containment of GMO, it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal)

New they are quite expensive, but I am sure we can find one on ebay, or to repair etc;

http://www.medisave.co.uk/clinical-waste-hands-free-bin-50-litre-yellow-lid-p-7466.html

http://www.wybone.co.uk/clinical-waste-bins/sackholders/

http://officebins.co.uk/pedal-medical-bins/open-body-hands-free-no-wheels-20-litre

http://www.nisbets.co.uk/4/Cleaning-and-Hygiene-Waste-Management-Bins-Sack-Trolley/c03c02c01c02.r10.1

Agarose

molecular biologists get through this stuff like there is no tomorrow. It's for making gels and stuff. It's also not that cheap...

SKU: NBS-AG500 £171.00

http://www.nbsbio.co.uk/nbs-ag500

500g 149.00 €

http://www.melford.co.uk/index.php?t=details&sid=\&&id=104

Lab consumables

large gloves

disposable pipettes

Sterile petri dishes

Gas can for bunsen burner