BioLab Manual: Difference between revisions
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Revision as of 14:19, 25 April 2014
Safety
Protocols
Established
The protocols we have got working in the Biohackspace:
- DNA extraction using Chelex 100
- DNA extraction and precipitation with ethanol / isopropanol
- Make and run an agarose gel
- Growing Escherichia coli
- Growing Vibrio fischeri (our first attempt)
Untested
The protocols we we would like to get working in the Biohackspace.
Media
Equipment
- Biobot
- Thermal Cycler
- Electrophoresis Power Supply
- Big centrifuge
- Small Centrifuge
- Gel Electrophoresis Box (How we made our own)
- Scales & Measures
- Autoclave
- Incubator
- Gilson Pipettes
- Microscope
- Various Consumables
Strains
Reagents
The reagents we currently have stocked:
- A little bit of Monica in my life
- A little bit of Erica by my side
- A little bit of Rita is all I need
- A little bit of Tina is what I see
- A little bit of Sandra in the sun
- A little bit of Mary all night long
- A little bit of Jessica here I am
- A little bit of you makes me your man
See also Project:Making our own reagents
Suppliers
- Sigma-Aldrich - Good for custom primers
- Cole-Parmer
- Farnell
- Chang bioscience - pipettes
- NBSBio - Good for ladders and cheap agarose (only low concentration) NB: Be sure to navigate to http://www.nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird. Standard shipping cost is £15
- Web scientific - Taq and ladder
- New England Biolabs They sell very cheap taq. We could also look at their restriction enzymes. However atm they won't sell to us as they only sell to universities or registered biolabs. See this post and this post. Once we have all our registrations for LBL sorted we could try again.
- Thermoscientific - Recommended for good value restriction enzymes. Not tried yet.
- VWR - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace.
- Invitrogen We failed their due diligence check. See post here and here. They have cheap TBE.
- IDT - possible source for primers. Haven't tried them yet.
- cheaplabstuff (ebay) - Cheap 1.5ml and 15ml tubes in small quantities. Also pasteur pipettes.
- microsupplies (ebay) - Cheap 5-200ul and 100-1000ul tips by the 1000
- Cambridge Bioscience - Not yet used, may supply GelRed or GelGreen (enquiry by Bene)
Also see general hackspace suppliers page.
