Good news - we're open for limited services in Wembley. Ujima House is now actively under refurbishment and we'd love your help in making the space the best it can be.

Please pay attention to the main LHS mailing list or pop into our #london-hack-space IRC channel and say hello.

During this interim period donations and continued membership are greatly appreciated while we transition to our new space.

BioLab Manual

From London Hackspace Wiki
Jump to: navigation, search

Safety

All users of the Biolab must follow the BioLab Usage Guidelines for their own and other user's safety.

Protocols

Instructions for (a "Blink LED" for easy first transformations)

Instructions for making general stock solutions

For more extensive databases of protocols:

Established Protocols

The protocols we have got working in the Biohackspace:

We also have protocols on our github SOPs page

Untested Protocols

The protocols we would like to get working in the Biohackspace.

Media Recipes

Equipment

Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
26 January 2015 20:22:21
by [[]]
Is maintained by;
Czechton
Doesn't require training
Unrestricted
Faulty
Last updated;
26 January 2015 20:19:42
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Good working order
Last updated;
23 August 2015 20:34:44
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Display is faulty, blinks on/off intermittently, Power supply itself seems fine
Last updated;
16 June 2015 17:38:35
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Good working order
Last updated;
30 January 2015 07:45:13
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Good working order
Last updated;
27 April 2014 18:13:41
by [[]]
Is maintained by;
Tolland
Doesn't require training
Unrestricted
Good working order
Last updated;
27 April 2014 14:42:07
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Good working order
Last updated;
15 December 2014 20:55:13
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Scrapped
Last updated;
20 October 2014 22:51:52
by [[]]
Is maintained by;
Biohackers
Requires training
Unrestricted
Good working order
Last updated;
1 May 2014 21:59:38
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
27 April 2014 14:40:38
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
27 April 2014 14:39:37
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
12 March 2016 23:58:38
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
13 October 2014 11:21:22
by [[]]
Is maintained by;
Biohackers, tolland
Doesn't require training
Unrestricted
Good working order
Last updated;
23 January 2016 16:16:05
by [[]]
Is maintained by;
Tolland
Requires training
Unrestricted
Not calibrated
Last updated;
2 January 2015 15:16:58
by [[]]
Is maintained by;
Biohackers
Doesn't require training
Unrestricted
Good working order
Last updated;
5 February 2015 13:34:49
by [[]]
Located in;
Ground Floor
No maintainer listed
Doesn't require training
Unrestricted
Shaker broken
Last updated;
1 July 2015 09:42:45
by [[]]
Located in;
NA
No maintainer listed
Training requirement unknown
Unknown ACnode status
Dismantled
Last updated;
11 February 2017 16:10:58
by [[]]
Located in;
Being Repaired
Is maintained by;
Biohackers
Requires training
Unrestricted
Borked
Last updated;
5 April 2015 06:36:44
by [[]]
No maintainer listed
Doesn't require training
Unknown ACnode status
Good working order
Last updated;
26 February 2015 21:06:36
by [[]]


To create a page for a piece of lab equipment fill out this form and make sure you put "Biohacking" as the subcategory.

We also have an array of glassware and consumables.

This site has a directory of service engineers for various biomedical/biochemical equipment:

http://www.labwrench.com/?equipment.requestAQuote/categoryNo/707/Mass-Spectrometers/

Strains

Reagents

Category:BiolabInventory

The reagents we currently have stocked

General Reagents
Reagent Brand Location
Bacto Agar Shelves
Bacto Yeast Extract Shelves
LB broth powder Shelves
TBE buffer x10 Shelves
Sodium Acetate Shelves
Ethidium Bromide Drawers
Tri-Sodium Citrate-2 Water Shelves
Magnesium Chloride Shelves
Calcium Chloride Shelves
Agarose Shelves
Glycerin Shelves
Ampicillin Sodium Salt (powdered) Fridge
Kanamycin Sulphate (powdered) Fridge
IPTG Freezer
Proteinase K 1mg/ml Freezer
DNA (Primers, Plasmids, etc.)
Reagent Description Brand
pUC19 control DNA NEB #N3041A NEB
Q5 Hi-GC enhancer 5x concentrate NEB
Restriction Enzymes
Reagent Cutting Site Brand

See also: Project:Making our own reagents


   link to shopping list G spreadsheet

Suppliers

  • Sigma-Aldrich - Good for custom primers
  • Cole-Parmer
  • Farnell
  • Chang bioscience - pipettes
  • NBSBio - Good for ladders and cheap agarose (only low concentration) NB: Be sure to navigate to http://www.nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird. Standard shipping cost is £15
  • Web scientific - Taq and ladder
  • New England Biolabs They sell very cheap taq. We could also look at their restriction enzymes. However atm they won't sell to us as they only sell to universities or registered biolabs. See this post and this post. Once we have all our registrations for LBL sorted we could try again.
  • Thermoscientific - Recommended for good value restriction enzymes. Not tried yet.
  • VWR - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace.
  • Invitrogen We failed their due diligence check. See post here and here. They have cheap TBE.
  • IDT - possible source for primers. Haven't tried them yet.
  • cheaplabstuff (ebay) - Cheap 1.5ml and 15ml tubes in small quantities. Also pasteur pipettes.
  • microsupplies (ebay) - Cheap 5-200ul and 100-1000ul tips by the 1000
  • Cambridge Bioscience - Not yet used, may supply GelRed or GelGreen (enquiry by Bene)

Also see general hackspace suppliers page.