Flow Cytometer: Difference between revisions
m (Czechton moved page Equipment/Flow Cytometer to Flow Cytometer) |
mNo edit summary |
||
| Line 13: | Line 13: | ||
| owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS --> | | owner = LHS/biohackers <!-- Provide a link to owners members page if other than LHS --> | ||
| origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki --> | | origin = Donated by Imperial College London<!-- If via pledge, please link to the completed pledge page on the wiki --> | ||
| location = | | location = In storage | ||
| maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. --> | | maintainers = tolland<!-- NO LINKS PLEASE; it currently breaks the template. If someone is nominated as managing the upkeep of this item, please list them here. --> | ||
| template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates --> | | template_ver = 1.1 <!-- Please do not change. Used for tracking out-of-date templates --> | ||
| Line 19: | Line 19: | ||
Imperial College donated a Flow Cytometer. The unit is | Imperial College donated a Flow Cytometer. The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) | ||
'''Status:''' | '''Status:''' We are going to dismantle it for parts. | ||
There are a number of large external units some of which are for providing power and cooling to the large laser. | There are a number of large external units some of which are for providing power and cooling to the large laser. | ||
There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html | There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html | ||
Revision as of 15:31, 16 June 2014
| Flow Cytometer | |
|---|---|
 | |
| Model | Coulter EPICS ALTRA |
| Sub-category | Biohacking |
| Status | Faulty |
| Consumables | Sheath Solution, Cleaning fluid, sample tubes |
| Accessories | Unknown |
| Training requirement | Yes |
| Training link | Unknown |
| Owner | LHS/biohackers |
| Origin | Donated by Imperial College London |
| Location | In storage |
| Maintainers | tolland |
Imperial College donated a Flow Cytometer. The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space)
Status: We are going to dismantle it for parts.
There are a number of large external units some of which are for providing power and cooling to the large laser.
There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
It was donated as working, but in need of repair. There is quite a lot of damage due to corrosion, and to the sampling area which we are working on.
The machine requires a dust free environment due to it's use of lasers and optics.
I am currently looking to obtain a copy of the service manual.
Educationz
Harvard University extension school course on microfluidics, course notes
http://isites.harvard.edu/icb/icb.do?keyword=k41912&pageid=icb.page213804
Caltech course notes on microfluidics
http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf
Cornell book on microfluidics
http://www.kirbyresearch.com/index.cfm/wrap/textbook/microfluidicsnanofluidics.html
Flow cytometry tutorial
This is a AV presentation describing the basics of the technology;
http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html
Practical Flow cytometry
This is an online version of the bible of Flow cytometry (Shapiro 2003)
http://www.coulterflow.com/bciflow/practical/book/index.html
Dako - flow cytometry
http://www.dako.com/08065_15dec05_guide_to_flow_cytometry.pdf
Microfluidics Links
Introduction to fluidics
