Difference between revisions of "Biohacking Wishlist"
m |
m |
||
| Line 22: | Line 22: | ||
"Microvolume UV-Vis spectrophotometers and fluorospectrometers" | "Microvolume UV-Vis spectrophotometers and fluorospectrometers" | ||
http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf | http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf | ||
| + | |||
| + | == Ultra Low Temp Freezer (-80C, -70C, or comparable) == | ||
| + | |||
| + | For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard. | ||
| + | |||
| + | Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells. | ||
== Coomassie brilliant blue == | == Coomassie brilliant blue == | ||
| Line 28: | Line 34: | ||
"The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia | "The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia | ||
| − | |||
* Coomassie Brilliant Blue 1 | * Coomassie Brilliant Blue 1 | ||
Revision as of 22:23, 4 August 2015
Visible Spectrophotometer for OD600
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison. Something like this would probably do; http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg
UV Spectrophotometer
this would also be useful, but unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP; https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb
mg Lab balance with RS-232/usb outputs
It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better; http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html
or possibly a UK supplier of something like this; http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm
Nanodrop spectrophotometer
"Microvolume UV-Vis spectrophotometers and fluorospectrometers" http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf
Ultra Low Temp Freezer (-80C, -70C, or comparable)
For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.
Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.
Coomassie brilliant blue
We currently need "Coomassie Brilliant Blue 1" to be able to perform Bradform protein assays
"The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia
- Coomassie Brilliant Blue 1
- Spectrophotometer and tubes
http://www.nbsbio.co.uk/cb0037-25g
http://en.wikipedia.org/wiki/Coomassie_Brilliant_Blue
Foot pedal operated lab waste bin
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.
one of these examples would be good;
http://www.medisave.co.uk/clinical-waste-hands-free-bin-50-litre-yellow-lid-p-7466.html
http://www.wybone.co.uk/clinical-waste-bins/sackholders/
http://officebins.co.uk/pedal-medical-bins/open-body-hands-free-no-wheels-20-litre
Lab consumables
large gloves
disposable pipettes
Sterile petri dishes
Gas can for bunsen burner
Agarose
For making gels and stuff. It's also not that cheap...
SKU: NBS-AG500 £171.00
http://www.nbsbio.co.uk/nbs-ag500
500g 149.00 €