Difference between revisions of "Biohacking Wishlist"

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== Spectrophotometer ==
+
== Lab Balance ==
  
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison.
+
We have several pieces of equipment that need calibrating.
 +
 
 +
== PCR machine ==
  
Unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP;
+
We currently have 1 working PCR machine, but it's quite old.
https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb
 
  
== Coomassie brilliant blue  ==
+
== Lab balance with RS-232/usb outputs ==
  
We currently need "Coomassie Brilliant Blue 1" to be able to perform Bradform protein assays
+
It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better;
 +
http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html
  
"The Bradford protein assay is a spectroscopic analytical procedure used to measure the concentration of protein in a solution. It is subjective, i.e., dependent on the amino acid composition of the measured protein. The Bradford protein assay was developed by Marion M. Bradford." wikipedia
+
or possibly a UK supplier of something like this;
 +
http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm
  
 +
== Visible Spectrophotometer for OD600 ==
  
*  <s>Lyophilized bovine plasma gamma globulin</s> received
+
A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison.
*    Coomassie Brilliant Blue 1
+
Something like this would probably do;
*    <s>0.15 M NaCl</s> in stores
+
http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg
*    Spectrophotometer and tubes
 
*    <s>Micropipettes</s> in stores
 
  
http://www.nbsbio.co.uk/cb0037-25g
+
== UV Spectrophotometer ==
  
http://en.wikipedia.org/wiki/Coomassie_Brilliant_Blue
+
this would also be useful, but unfortunately replacement deuterium bulbs are expensive.  seem like at least 100GBP;
 +
https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb
  
==Foot pedal operated lab waste bin==
 
  
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal)
 
  
<gallery>
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== Nanodrop spectrophotometer ==
File:Open Frame Sackholders MAIN.jpg
 
</gallery>
 
  
one of these examples would be good;
+
"Microvolume UV-Vis spectrophotometers and fluorospectrometers"
 +
http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf
  
http://www.medisave.co.uk/clinical-waste-hands-free-bin-50-litre-yellow-lid-p-7466.html
+
== Ultra Low Temp Freezer (-80C, -70C, or comparable) ==
  
http://www.wybone.co.uk/clinical-waste-bins/sackholders/
+
For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.
  
http://officebins.co.uk/pedal-medical-bins/open-body-hands-free-no-wheels-20-litre
+
Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.
  
http://www.nisbets.co.uk/4/Cleaning-and-Hygiene-Waste-Management-Bins-Sack-Trolley/c03c02c01c02.r10.1
+
==<s>Foot pedal operated lab waste bin</s>==
 +
(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!)
  
 +
In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.
  
 +
<gallery>
 +
File:Open Frame Sackholders MAIN.jpg
 +
</gallery>
  
 
==Lab consumables==
 
==Lab consumables==

Latest revision as of 20:24, 13 November 2015

Lab Balance

We have several pieces of equipment that need calibrating.

PCR machine

We currently have 1 working PCR machine, but it's quite old.

Lab balance with RS-232/usb outputs

It would be useful to be able to calibrate the liquid handling systems using an automated system. Currently we don't have anything like that. A 1 mg would probably be sufficient, but something like this is better; http://www.atp-instrumentation.co.uk/83g-x-0-0001g-0-1mg-rs-232-analytical-balance-1.html

or possibly a UK supplier of something like this; http://www.scalesgalore.com/Adam_Equipment_hcb_Balances.cfm

Visible Spectrophotometer for OD600

A modern working Spectrophotometer would be a great asset to the lab. We are currently making do with guess-work, and Macfarland standards for comparison. Something like this would probably do; http://www.coleparmer.co.uk/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UY-83055-10?referred_id=3482&gclid=CJeN5M-nkMcCFUzHtAodMaEORg

UV Spectrophotometer

this would also be useful, but unfortunately replacement deuterium bulbs are expensive. seem like at least 100GBP; https://www.google.co.uk/search?q=spectropphoeter+deuterium#newwindow=1&tbm=shop&q=deuterium+bulb


Nanodrop spectrophotometer

"Microvolume UV-Vis spectrophotometers and fluorospectrometers" http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf

Ultra Low Temp Freezer (-80C, -70C, or comparable)

For storage of various samples and cells, a ULT freezer (usually -80C, but that's possibly over spec for our requirement) is the industry standard.

Currently it appears that both the initial outlay and the power costs for running this equipment are beyond our budget. But its a would-like to be able to make up large batches of competent cells.

Foot pedal operated lab waste bin

(Edit: Edo sourced a couple of these from UCL's warp account.. Thanks!)

In order to improve the general hygiene it is recommended to use Hands free laboratory rubbish bins, (the ones that are operated with a foot pedal) The lids stops insects getting in, and waste escaping out, and the pedal saves you from contaminating your PPE gloves.

Lab consumables

large gloves

disposable pipettes

Sterile petri dishes

Gas can for bunsen burner

Agarose

For making gels and stuff. It's also not that cheap...

SKU: NBS-AG500 £171.00

http://www.nbsbio.co.uk/nbs-ag500

500g 149.00 €

http://www.melford.co.uk/index.php?t=details&sid=\&&id=104