Flow Cytometer

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Flow Cytometer
IMG 20130927 144739.jpg
Model Coulter EPICS ALTRA
Sub-category Biohacking
Status Scrapped
Consumables Sheath Solution, Cleaning fluid, sample tubes
Accessories Unknown
Training requirement Yes
Training link Unknown
Owner LHS/biohackers
Origin Donated by Imperial College London
Location In storage
Maintainers tolland

Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.

Status: It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.

Status: The machine was too expensive to repair, and was too large to store and therefore has been scrapped. Several of the parts have been incorporated into other projects, or have been ebayed.

The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.


There are a number of large external units some of which are for providing power and cooling to the large laser.

There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

The machine requires a dust free environment due to it's use of lasers and optics.

I am currently looking to obtain a copy of the service manual.


Educationz

Harvard University extension school course on microfluidics, course notes

http://isites.harvard.edu/icb/icb.do?keyword=k41912&pageid=icb.page213804

Caltech course notes on microfluidics

http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf


Cornell book on microfluidics

http://www.kirbyresearch.com/index.cfm/wrap/textbook/microfluidicsnanofluidics.html


Flow cytometry tutorial

This is a AV presentation describing the basics of the technology;

http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html


Practical Flow cytometry

This is an online version of the bible of Flow cytometry (Shapiro 2003)

http://www.coulterflow.com/bciflow/practical/book/index.html


Dako - flow cytometry

http://www.dako.com/08065_15dec05_guide_to_flow_cytometry.pdf

Microfluidics Links

Introduction to fluidics

http://www.youtube.com/watch?v=y1waCEPpWPI