Flow Cytometer

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Flow Cytometer
IMG 20130927 144739.jpg
Model Coulter EPICS ALTRA
Sub-category Biohacking
Status Faulty
Consumables Sheath Solution, Cleaning fluid, sample tubes
Accessories Unknown
Training requirement Yes
Training link Unknown
Owner LHS/biohackers
Origin Donated by Imperial College London
Location In storage
Maintainers tolland


Imperial College donated a Flow Cytometer. It was donated as working, but in need of repair. However there is a lot of damage due to corrosion, which might be impossible to fix for a reasonable amount of time and money.

The unit is too large to be stored in the biolab (1.8m X 0.8m = 1.44 square meters of floor space) and was previously moved to storage while we worked on it.

Status: It's sustained damage to the sampling area, and we are missing some bits. Repairing is likely to be very $$$, so we are planning to dismantle it and use the optics and lasers on other projects.

There are a number of large external units some of which are for providing power and cooling to the large laser.

There is a good description of how a flow cytometer works here; http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html

The machine requires a dust free environment due to it's use of lasers and optics.

I am currently looking to obtain a copy of the service manual.


Educationz

Harvard University extension school course on microfluidics, course notes

http://isites.harvard.edu/icb/icb.do?keyword=k41912&pageid=icb.page213804

Caltech course notes on microfluidics

http://thesis.library.caltech.edu/4796/3/02_Chapter_2.pdf


Cornell book on microfluidics

http://www.kirbyresearch.com/index.cfm/wrap/textbook/microfluidicsnanofluidics.html


Flow cytometry tutorial

This is a AV presentation describing the basics of the technology;

http://media.invitrogen.com.edgesuite.net/tutorials/4Intro_Flow/player.html


Practical Flow cytometry

This is an online version of the bible of Flow cytometry (Shapiro 2003)

http://www.coulterflow.com/bciflow/practical/book/index.html


Dako - flow cytometry

http://www.dako.com/08065_15dec05_guide_to_flow_cytometry.pdf

Microfluidics Links

Introduction to fluidics

http://www.youtube.com/watch?v=y1waCEPpWPI