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BioLab Manual: Difference between revisions
From London Hackspace Wiki
→Protocols
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= Protocols = | = Protocols = | ||
== Established == | Instructions for [[Stock Solutions|making general stock solutions]] | ||
For more extensive databases of protocols: | |||
* http://www.protocolpedia.com/index.php | |||
* http://openwetware.org/wiki/Protocols | |||
== Established Protocols == | |||
The protocols we have got working in the Biohackspace: | The protocols we have got working in the Biohackspace: | ||
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* [[DNA extraction and precipitation with ethanol / isopropanol]] | * [[DNA extraction and precipitation with ethanol / isopropanol]] | ||
* [[Make and run an agarose gel]] | * [[Make and run an agarose gel]] | ||
* Growing Escherichia coli | * [[Growing Escherichia coli]] | ||
* Growing Vibrio fischeri ([[Project: Growing bacteria|our first attempt]]) | * [[Growing Vibrio fischeri]] ([[Project: Growing bacteria|our first attempt]]) | ||
* [[PCR]] | |||
The protocols | == Untested Protocols== | ||
The protocols we would like to get working in the Biohackspace. | |||
* [[E. coli GFP transformation]] | * [[E. coli GFP transformation]] | ||
== Media == | == Media Recipes == | ||
* [[LB Medium]] | |||
* [[BOSS Medium]] | |||
* [[SH Medium]] | |||
= Equipment = | = Equipment = | ||
