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CL1 BSO SOP GLP: Difference between revisions
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'''HEPA flow cabinet/area''' | '''HEPA flow cabinet/area''' | ||
The HEPA filtered laminar flow unit allows us to work in sterile air in order to prevent contamination of our work such as petri dishes and broths with other microorganisms. Proper usage of the laminar flow area is described in the SOPs. It should be noted that the laminar flow functions in such a way as to protect the user’s work rather than the user and so caution still be taken by the user to maintain the aseptic conditions of anything used within the flow area and in disposal of consumables to assist in containment of biomaterial. | |||
See SOPs on aseptic technique. | |||
'''Hot plate/Steam cooker/steriliser/autoclave''' | '''Hot plate/Steam cooker/steriliser/autoclave''' | ||
The sterilisation unit is used to destroy any potential microorganisms that might contaminate media, reagents and consumables to be used aseptically in micro and molecular biological procedures. High temperature and pressure is used to kill contaminants and the main risk to the user is from the heat of the metal pressurised unit during sterilisation and any vented steam. The system itself is unpowered and is heated using the hot plate which itself becomes hot enough to cause serious burns. Thermal gloves are available for handling the autoclave and material processed by it. It is recommended that the autoclave and contents are allowed to cool for a while before handling. | |||
The correct procedures for using the autoclave/steriliser to prepare media and destroy GM waste are described in the SOPs. | |||
'''Sink area''' | '''Sink area''' | ||
