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CL1 BSO SOP GLP: Difference between revisions
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'''Sink area''' | '''Sink area''' | ||
The sink area should remain clear. All glassware should be kept clean and out of the way. | |||
'''Chemicals and Storage''' | '''Chemicals and Storage''' | ||
As per chemical list, and MSDS by chemical | |||
'''Incubator''' | '''Incubator''' | ||
While the incubator itself doesn’t pose much risk due to typically operating in the temperature required for incubating bacteria care should be taken when moving samples to and from the incubator as per handling biomaterial mentioned above. | |||
'''Centrifuges''' | '''Centrifuges''' | ||
''' | The laboratory has a number of centrifuges available for use. The Jouan is a larger devide which can take 50ml tubes and larger containers if fitted with buckets and can spin up to 10,000 rpm. The Henle and MSE microcentaur can spin up to higher rpm with smaller samples. These centrifuges will not operate in their normal state without a closed lid preventing the user from coming into contact with the moving parts. | ||
There are two blood centrifuges which spin in the region of 1000 to 2000 rpm while much slower can be operated with open lids however this is strictly advised against for safety reasons. | |||
All centrifuges should be cleaned after use for the purpose of biosafety and containment as samples centrifuged at high speeds can leak producing contamination. | |||
'''Refrigerator, Freezer and Sample Storage''' | |||
The refrigerator maintains samples and reagents in the area of 4degC such as bacterial plates, and reagents for transformation. Due to the presence of biomaterial in the refrigerator caution should be exercised to prevent contamination of the user. | |||
The freezer contains longer term bacterial stocks and enzymatic reagents and similar caution should be taken. | |||
Specific details for the handling of the various chemicals and reagents stored in the freezer and refrigerator can be found in the chemicals and storage list above. | |||
Specific SOPs cover the storage and recovery of frozen bacterial stocks and procedures requiring the usage of frozen and refrigerated reagents. | |||
