Group:Biohacking: Difference between revisions

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Right now we're working on genetic testing, identifying specific genes using PCR and electrophoresis. We've been working mainly on [[sex typing with amelogenin]] and [[Blood typing]]. In August and September we worked with the [http://2012.igem.org/Team:University_College_London/HumanPractice/DIYbio UCL igem team] to develop a 'public biobrick'.
Right now we're working on genetic testing, identifying specific genes using PCR and electrophoresis. We've been working mainly on [[sex typing with amelogenin]] and [[Blood typing]]. In August and September we worked with the [http://2012.igem.org/Team:University_College_London/HumanPractice/DIYbio UCL igem team] to develop a 'public biobrick'.


* [[iGEM 2014]]
* [[Public Biobrick]] (in collaboration with the UCL igem team)
* [[Public Biobrick]] (in collaboration with the UCL igem team)
* [[Sex typing with amelogenin]]
* [[Sex typing with amelogenin]]

Revision as of 13:23, 16 February 2014

We are the biohacking or DIY biology group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved! It's fascinating, the field and community is growing all the time, together with the ability of amateurs to do cool stuff. Over the past year we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short local news video showing our lab and some of what we do in it.

List of biohacker wiki pages.

We also maintain an outward facing website at BioHackspace.org

How to use this wiki

This wiki is intended to be our main place where we share information on our group and our projects. If you want to go to our main, non-wiki, public facing site, go here To see results, procedures and some discussion of our projects go to the individual project pages. For more general techniques and procedures see the protocols section. For information on getting hold of equipment and reagents, see the here. Please feel free to add or improve any information, it is a wiki after all...

Our current projects / schedule

We're currently working on getting competent in various techniques of synthetic biology: DNA extraction, PCR, gel electrophoresis, ligation and transformation. Once we can do this, and have been certified as a class 1 lab, we plan to start synbio experiments. Right now we're working on genetic testing, identifying specific genes using PCR and electrophoresis. We've been working mainly on sex typing with amelogenin and Blood typing. In August and September we worked with the UCL igem team to develop a 'public biobrick'.

Schedule

  • First half of 2013 - If hackspace move goes ahead, secure a larger area for biohacking, and get the H&S approval to do GM work there. Then do GM work! To start off - transformer protocol, genomikon kit, then GFP.

People

Andy, Ben, Jim, Joel, Leo, Lui, Mike, Nicholas, Paddy, Paul, Raphael, Simon, Taylor, Tom, Tonderai, Will, Hamilton

How to find us

  1. Come to the Hackspace Open Evening - every tuesday from 7:30pm. There should be a few of us around to show you the lab and give you an idea of what we do. If you want to make sure we find each other you can email us to let us know you're coming.
  2. Email us here. You can also sign up to our mailing list where we do most of our communication here (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the google groups interface.

Membership

We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Most of us are paying members of the hackspace, you can find out how to become a hackspace member here. However it's not essential to become a member just to come along initially and find out what we do.

We also have a special membership for biohacking. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. To find out how to pay biohacking membership, contact us

Our lab

Biohacking lab

Our standard protocols / how to's

Our reagents + equipment

  • Wet stuff: our inventory of primers, buffers, stains...
  • Thermal Cycler (PCR Machine) Perkin Elmer 480
  • Gel electrophoresis box
  • Big centrifuge
  • Variable V/I electrophoresis power supply.
  • Incubator with shaker
  • Two centrifuges - speed ~1400 rpm. Max capacity about 15ml.
  • One microcentrifuge: 6000 or 14000 rpm. For 1.5ml tubes. Can also take 0.5ml tubes with washers.
  • Various flasks and beakers
  • Scale down to 0.1g
  • 1-10ul pipette + tips. Tips available for £27.60 from NBSbio
  • 5-50ul pipette + tips. Tips from NBSBio or from microsupplies
  • 100-1000ul pipette
  • Pasteur pipettes (50 for £3 from cheaplabstuff)
  • 0.5ml PCR tubes. 1000 for £24 from nbsbio
  • 1.5ml tubes. 100 for £3 from ebay or 1000 from NBSbio.
  • Pressure cooker (goes up to 15PSI and 121°C (this is industry standard, not actually tested) to be used as autoclave. Autoclave tape.

Equipment Pledges

Shopping list (See wet stuff for sources and prices not listed here)

  • 1-10ul tips (quite a few left but running low)
  • 100-1000ul pipette tips for new pipette. Microsupplies sells 1000 for £8.90 + shipping
  • More autoclavable glass beakers, conical flasks tubes and petri dishes.
  • blender for ice crushing
  • 70% ethanol spray
  • IPTG
  • Kanamycin
  • Microscope (Tom's is

http://www.microscopenet.com/40x2000x-binocular-biological-compound-microscope-p-9100.html)

  • Thermometer for liquids

Suppliers

  • Sigma-Aldrich - Good for custom primers
  • Cole-Parmer
  • Farnell
  • Chang bioscience - pipettes
  • NBSBio - Good for ladders and cheap agarose (only low concentration) NB: Be sure to navigate to http://www.nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird. Standard shipping cost is £15
  • Web scientific - Taq and ladder
  • New England Biolabs They sell very cheap taq. We could also look at their restriction enzymes. However atm they won't sell to us as they only sell to universities or registered biolabs. See this post and this post. Once we have all our registrations for LBL sorted we could try again.
  • Thermoscientific - Recommended for good value restriction enzymes. Not tried yet.
  • VWR - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace.
  • Invitrogen We failed their due diligence check. See post here and here. They have cheap TBE.
  • IDT - possible source for primers. Haven't tried them yet.
  • cheaplabstuff (ebay) - Cheap 1.5ml and 15ml tubes in small quantities. Also pasteur pipettes.
  • microsupplies (ebay) - Cheap 5-200ul and 100-1000ul tips by the 1000
  • Cambridge Bioscience - Not yet used, may supply GelRed or GelGreen (enquiry by Bene)

Also see general hackspace suppliers page.

Wish list

Main article: Biohacking/Wishlist

Resources

Education

A primer for synthetic biology

Community

PCR primer design

Proposed code of conduct

See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct

FAQ about DIYBio

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