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== Biohacking / DIYBio at LHS ==
{{Group
|logo=Biohack_logo.png
|name=Biohacking
|when=Every Wednesday
|what=
|time=19:30
|recurring=1}}


We're keen to get the nascent field of amateur biology going in the London Hackspace. Over the coming months we'll be developing research equipment, optimising techniques and running some research projects of our own. Most of the people involved aren't starting with a biology background, so get involved!
We are the '''biohacking''' or '''DIY biology''' ([http://openwetware.org/wiki/DIYbio/FAQ Official FAQ about DIY-Biology]) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved!


http://en.wikipedia.org/wiki/User:Cathalgarvey/Books/A_Beginner's_Guide_to_Biotechnology
Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short [http://www.bbc.co.uk/news/uk-england-london-19964886 local news video] showing our (previous, smaller) lab and some of what we do in it.


== Events ==
We will soon be meeting every month, check back here for our 2019 restart.
[[User:Bugs | Bugs]] and Sara ran an [[Biohack_workshop|Introduction to Biohacking]] workshop, giving a crash course in molecular biology and a great overview of what biohackers around the world are getting up to. The talks were recorded and are now on YouTube: [[http://www.youtube.com/watch?v=9W5ZshwteUw Intro to mol. biol]], [[http://www.youtube.com/watch?v=muxDfkKDXnY Groups around the world]].
We have a list of [[Biohacking FAQ|frequently-asked questions]].


We also gave condensed versions of these talks at the Science Museum's Dana Centre, which seems to have netted us a few new members. Awesome.
[[:Category:Biohacking|'''List of biohacker wiki pages.''']]
 
We also maintain an outward facing website at [http://biohackspace.org/ BioHackspace.org]
 
== How to find us ==
# Email us [mailto:hello@biohackspace.org here]. You can also sign up to our mailing list where we do most of our communication [https://lists.kentgeek.org/mailman/listinfo/lhs-biohacking here] (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the [https://groups.google.com/forum/?fromgroups#!forum/london-biohackers google groups] interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at [http://webchat.freenode.net/?channels=london-biohackers #london-biohackers on freenode]
 
== Biohackspace Membership ==
We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member [https://london.hackspace.org.uk/signup.php here].
 
There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay.  Details on how to setup payment to become a biohackspace member are here: [[London Biohackers Membership | Details of Membership]].
 
We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact [mailto:hello@biohackspace.org here by email], or see the [[Remote Membership|Donations/Remote members]] page for more details.
 
However it's not essential to become a member just to come along initially and find out what we do.
 
== Organization ==
 
The biohackspace operates as a Company Limited by guarantee. The legal details of this are [[London Biological Laboratories|here]]


== Projects ==
== Projects ==
*DNA forensics
We are currently well equipped to carry out molecular biology experiments.  The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley.
*[[Project:Biohacking/Algal_biodiesel|Algal biodiesel]]
*[[Sex typing with amelogenin]]


What to do after Amelogenin?
=== Current Projects ===
* Hacking the lab
* * Recommissioning RO water system
* * Recommissioning Laminar Flow system
* * Building Laminar Flow System into a hood
* Culturing Local Algae
* Lab Automation
* Stirling Engine for Cryogenics


[[http://en.wikipedia.org/wiki/List_of_Mendelian_traits_in_humans List_of_Mendelian_traits_in_humans]]
=== Completed Projects ===


[[http://en.wikipedia.org/wiki/MC1R MC1R]]
* [http://2015.igem.org/Team:London_Biohackspace iGEM 2015]
* [[Project:iGEM 2014]]
* [[Project:Flow Hood]]
* [[E. coli transformation]]
* [[Image Focus Stacking]]
* [[CL1_BSO_SOP_GLP|Lab Containment Level 1]]
* [[Project:Growing bacteria|Growing V. fischeri]]
* [[Project:Sex typing with amelogenin]]
* [[Project:Public Biobrick]] (in collaboration with the UCL igem team)
* [[Blood typing]]
* [[Project:Plant species testing]]
* [[Project:Gel electrophoresis box]]
* [[Project:Shaker platform]]


[[http://en.wikipedia.org/wiki/Rhd Rhd]]
=== Future Projects ===
* [[Project:Making our own reagents]]
*[[MindControlBacteria]]
* More and more becoming current project :)
Add your own!


[[http://en.wikipedia.org/wiki/Mendelian_Inheritance_in_Man Mendelian_Inheritance_in_Man]]
== Our lab ==
Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a [[Lab|floor plan of the lab here]].


[http://www.ncbi.nlm.nih.gov/pubmed/9493346 What was in those brownies, anyway?]
We <strike> are currently working towards being </strike> are a certified Containment Level 1 Lab ([[BioSafety|more details on biosafety classifications]]).


==Lab Notebook Entries==
The Lab is the registered office of our limited company, [[London Biological Laboratories]], used to deal with suppliers.
*13-04-2011 [[Project:Biohacking/20110413|First attempt at electrophoresis of DNA on agarose gel]]
 
*11-05-2011 [[Project:Biohacking/20110511|Gene detection trial run: Cheek swab -> PCR -> Electrophoresis]]
=== How to use the Lab ===
Please do not touch things in the lab if you do not know what they are or how to use them. Many items may be hazards to your health if used incorrectly.
 
Our [[BioLab Manual|lab manual]] details our equipment, reagents, strains and our established protocols.
 
=== Starting your own lab? ===
We have some notes on [[Starting your own biolab|starting your own biolab]] based on our experiences.
 
=== Equipment we made ===
Our hope is to be able to use self-developed or open source hardware equipment where possible in order to carry out our projects. Here is what we have done so far:
 
* [[Project:Gel electrophoresis box]]
* [[Biobot]]
* [[Project:Shaker platform]]
 
A list of biohacking hardware projects is here [[Project:Biohardware]]
 
=== Equipment Pledges ===
 
[[Biohacking Wishlist]]
 
* [[Pledge: Flow Hood]]
* [[Pledge:Lab Microscope]]
* [[Pledge:vacum forming machine]]
* [[Pledge:Gluconacetobacter_hansenii]]


== Resources ==
== Resources ==
Line 35: Line 105:
*[http://www.nature.com/nature/comics/syntheticbiologycomic/ Nature.com comic about Synthetic Biology] - Nice introduction to playing with genetic engineering/cloning, though aimed at kids
*[http://www.nature.com/nature/comics/syntheticbiologycomic/ Nature.com comic about Synthetic Biology] - Nice introduction to playing with genetic engineering/cloning, though aimed at kids


*[http://www.youtube.com/watch?v=9W5ZshwteUw Bugs' Crash Course in Molecular Biology] - Recorded at the hackspace
*[http://www.youtube.com/watch?v=9W5ZshwteUw Bugs' Crash Course in Molecular Biology] - Recorded at the hackspace. [http://wiki.london.hackspace.org.uk/view/File:Intro_to_BioHacking.pdf Slides]
*[http://www.youtube.com/watch?v=muxDfkKDXnY Sara's Introduction the the Biohacking Community] - Recorded at the hackspace
*[http://www.youtube.com/watch?v=muxDfkKDXnY Sara's Introduction the the Biohacking Community] - Recorded at the hackspace
 
[http://diyhpl.us/~bryan/papers2/bio/books/Primer%20for%20Synthetic%20Biology.pdf A primer for synthetic biology]
*[http://learn.genetics.utah.edu/content/labs/gel/ Good introduction to gel electrophoresis] - Explaining one of the core techniques for working with DNA
*[http://learn.genetics.utah.edu/content/labs/gel/ Good introduction to gel electrophoresis] - Explaining one of the core techniques for working with DNA
*[http://learn.genetics.utah.edu/content/labs/pcr/ Good introduction to the  Polymerase Chain Reaction (PCR)] - Explaining one of the core techniques for working with DNA
*[http://learn.genetics.utah.edu/content/labs/pcr/ Good introduction to the  Polymerase Chain Reaction (PCR)] - Explaining one of the core techniques for working with DNA
*[http://www.nature.com/news/2010/101006/full/467650a.html Nature.com article on Biohacking] - Free access. Nice explanation of what's needed in a lab and how different groups are managing it.
*[http://www.nature.com/news/2010/101006/full/467650a.html Nature.com article on Biohacking] - Free access. Nice explanation of what's needed in a lab and how different groups are managing it.
*[[Bioinformatics_resources | Bioinformatics Resources]]
*[http://wiki.london.hackspace.org.uk/w/images/4/43/Biohacking_workshop_Aug2012.pdf Slides] from Bioinformatics workshop August 2012
*[http://en.wikipedia.org/wiki/User:Cathalgarvey/Books/A_Beginner's_Guide_to_Biotechnology Cathal Garvey's beginner's guide to biotech]
*[https://docs.google.com/drawings/d/1hXO4uu_TZ_yI3amJ1jGo9zu2XU3gX9yXMchC9gGZT6k/edit Flowchart diagram of DNA extraction protocol]


*[[Bioinformatics_resources | Bioinformatics Resources]]
=== Community ===
=== Community ===
*[http://diybio.org/blog Interesting DIYBio community blog] - Interesting blog taking articles from a few different groups
*[http://diybio.org/blog Interesting DIYBio community blog] - Interesting blog taking articles from a few different groups
*[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established bihacking group in California
*[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established biohacking group in California
*[http://www.tmplab.org/wiki/index.php/Brain_Computer_Interface Brain-Computer interface at Paris hackspace]
*[http://www.tmplab.org/wiki/index.php/Brain_Computer_Interface Brain-Computer interface at Paris hackspace]
*[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1077.html Nature.com article about biohacking community] - Not the same one as above. Interesting, but behind a paywall :(.
*[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1077.html Nature.com article about biohacking community] - Not the same one as above. Interesting, but behind a paywall :(.
** you can access it here: [http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf] -- [http://heybryan.org/ kanzure] [[Special:Contributions/70.114.205.110|70.114.205.110]] 19:40, 2 January 2011 (UTC)
** you can access it here: [http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf] -- [http://heybryan.org/ kanzure] [[Special:Contributions/70.114.205.110|70.114.205.110]] 19:40, 2 January 2011 (UTC)
=== Equipment ===
*[[Biohacking/Equipment/Thermal_Cycler_(Perkin_Elmer_480) | Thermal Cycler (PCR Machine); Perkin Elmer 480]]
*[http://openpcr.org/ Open Source, Hackable PCR machine] - We'll probably buy one of these when they become available
*[http://www.russelldurrett.com/lightbulbpcr.html Light Bulb PCR] " less than $50 to build this machine (including the $30 arduino)"
*[http://www.pearlbiotech.com/ Open Source Electrophoresis tanks] - They sell kits, but we can probably just use their published plans to laser-cut our own. Also [http://learn.genetics.utah.edu/content/labs/gel/gelchamber/]
*[http://www.thingiverse.com/thing:1483 Dremelfuge] - microcentrifuge to be made on a 3D printer. We have one, but using it is a bit ... character building. Need to print a new one.
*[[Biohacking/Wetstuff | Wet stuff]] Wet stuff: our inventory of primers, buffers, stains...
=== PCR primer design ===
* http://www.cybertory.org/exercises/primerDesign/index.html
* http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html
* http://www.premierbiosoft.com/primerdesign/index.htm
* http://molbiol-tools.ca/PCR.htm
* http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome
* http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi
* http://bibiserv.techfak.uni-bielefeld.de/genefisher2/
=== Suppliers ===
Cf. [[Suppliers]].
* [http://www.sigmaaldrich.com/united-kingdom.html Sigma-Aldrich] - Don't seem to want to sell to us
* [http://www.coleparmer.co.uk/ Cole-Parmer]
* [http://uk.farnell.com/ Farnell]
* [http://www.nbsbio.co.uk/ NBSBio] - Order placed with them, lets see whether they deliver... [NB: Be sure to navigate to http://'''www.'''nbsbio.co.uk NOT http://nbsbio.co.uk, or their payment system doesn't work. Weird.]
* [http://www.neb.com New England Biolabs] Free sample of Taq polymerase requested, let's see whether they deliver...
* [http://www.vwr.com VWR]] - Big supplier of chemicals, glassware and various lab stuff. Sounds very positive about selling to the hackspace. [[User:Bugs|Bugs]] is in the process of setting up an account with them.
=== Contact ===
If you want to chat about biohacking, post to the [https://lists.kentgeek.org/mailman/listinfo/lhs-biohacking Biohacking Mailing List] or contact one of us directly :).
== Pledges ==
Assorted biohacking pledges can be found [[Pledges/Biohacking| here]].
Current Pledges:
* Gene detection experiment kit


== Proposed code of conduct ==
== Proposed code of conduct ==
See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct
See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct


== FAQ ==
[[Category:Biohacking]]
* [http://openwetware.org/wiki/DIYbio/FAQ official do-it-yourself biology FAQ]
[[Category:Groups]]
 
== News ==
* [http://bit.ly/diybionews DIYbio-in-the-news is listed on the FAQ]..
 
== Electrophoresis box ==
 
* 7 / Apr / 2011: Photos from the outer box construction are here: http://lardcave.net/biohacking/
* 13 April 2001: Photos of the [http://www.flickr.com/photos/dekstop/sets/72157626368045215/ first Electrophoresis experiment]
 
We've made one. Measurements for the outer box, in mm:
    Width: 102.45 to 102.9 (not a perfect glue job)
    Height to top (container): 31.8
    Height to top holes (for electrodes): 22.16
    Depth: about 171
    Thickness (of the perspex): about 3.2
 
Measurements for the inner box (which we ended up using, roughly):
    Width: 100
    Height to top: 20
    Depth: 100
 
So that is two panels measuring 100 x 20 (for the sides), and one panel measuring 100 x 100 (for the bottom).

Latest revision as of 06:16, 1 May 2019

Biohacking
Biohack logo.png
When Every Wednesday, from 19:30
Members

We are the biohacking or DIY biology (Official FAQ about DIY-Biology) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved!

Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short local news video showing our (previous, smaller) lab and some of what we do in it.

We will soon be meeting every month, check back here for our 2019 restart. We have a list of frequently-asked questions.

List of biohacker wiki pages.

We also maintain an outward facing website at BioHackspace.org

How to find us

  1. Email us here. You can also sign up to our mailing list where we do most of our communication here (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the google groups interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at #london-biohackers on freenode

Biohackspace Membership

We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member here.

There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. Details on how to setup payment to become a biohackspace member are here: Details of Membership.

We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact here by email, or see the Donations/Remote members page for more details.

However it's not essential to become a member just to come along initially and find out what we do.

Organization

The biohackspace operates as a Company Limited by guarantee. The legal details of this are here

Projects

We are currently well equipped to carry out molecular biology experiments. The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley.

Current Projects

  • Hacking the lab
  • * Recommissioning RO water system
  • * Recommissioning Laminar Flow system
  • * Building Laminar Flow System into a hood
  • Culturing Local Algae
  • Lab Automation
  • Stirling Engine for Cryogenics

Completed Projects

Future Projects

Add your own!

Our lab

Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a floor plan of the lab here.

We are currently working towards being are a certified Containment Level 1 Lab (more details on biosafety classifications).

The Lab is the registered office of our limited company, London Biological Laboratories, used to deal with suppliers.

How to use the Lab

Please do not touch things in the lab if you do not know what they are or how to use them. Many items may be hazards to your health if used incorrectly.

Our lab manual details our equipment, reagents, strains and our established protocols.

Starting your own lab?

We have some notes on starting your own biolab based on our experiences.

Equipment we made

Our hope is to be able to use self-developed or open source hardware equipment where possible in order to carry out our projects. Here is what we have done so far:

A list of biohacking hardware projects is here Project:Biohardware

Equipment Pledges

Biohacking Wishlist

Resources

Education

A primer for synthetic biology

Community

Proposed code of conduct

See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct

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