Difference between revisions of "Group:Biohacking"

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Latest revision as of 06:16, 1 May 2019

Biohacking

When	Every Wednesday, from 19:30
Members

We are the biohacking or DIY biology (Official FAQ about DIY-Biology) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved!

Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short local news video showing our (previous, smaller) lab and some of what we do in it.

We will soon be meeting every month, check back here for our 2019 restart. We have a list of frequently-asked questions.

List of biohacker wiki pages.

We also maintain an outward facing website at BioHackspace.org

How to find us

Email us here. You can also sign up to our mailing list where we do most of our communication here (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the google groups interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at #london-biohackers on freenode

Biohackspace Membership

We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member here.

There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. Details on how to setup payment to become a biohackspace member are here: Details of Membership.

We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact here by email, or see the Donations/Remote members page for more details.

However it's not essential to become a member just to come along initially and find out what we do.

Organization

The biohackspace operates as a Company Limited by guarantee. The legal details of this are here

Projects

We are currently well equipped to carry out molecular biology experiments. The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley.

Current Projects

Hacking the lab
* Recommissioning RO water system
* Recommissioning Laminar Flow system
* Building Laminar Flow System into a hood
Culturing Local Algae
Lab Automation
Stirling Engine for Cryogenics

Completed Projects

iGEM 2015
Project:iGEM 2014
Project:Flow Hood
E. coli transformation
Image Focus Stacking
Lab Containment Level 1
Growing V. fischeri
Project:Sex typing with amelogenin
Project:Public Biobrick (in collaboration with the UCL igem team)
Blood typing
Project:Plant species testing
Project:Gel electrophoresis box
Project:Shaker platform

Future Projects

Project:Making our own reagents
MindControlBacteria
More and more becoming current project :)

Add your own!

Our lab

Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a floor plan of the lab here.

We ~~are currently working towards being~~ are a certified Containment Level 1 Lab (more details on biosafety classifications).

The Lab is the registered office of our limited company, London Biological Laboratories, used to deal with suppliers.

How to use the Lab

Please do not touch things in the lab if you do not know what they are or how to use them. Many items may be hazards to your health if used incorrectly.

Our lab manual details our equipment, reagents, strains and our established protocols.

Starting your own lab?

We have some notes on starting your own biolab based on our experiences.

Equipment we made

Our hope is to be able to use self-developed or open source hardware equipment where possible in order to carry out our projects. Here is what we have done so far:

A list of biohacking hardware projects is here Project:Biohardware

Equipment Pledges

Biohacking Wishlist

Resources

Education

Nature.com comic about Synthetic Biology - Nice introduction to playing with genetic engineering/cloning, though aimed at kids

Bugs' Crash Course in Molecular Biology - Recorded at the hackspace. Slides
Sara's Introduction the the Biohacking Community - Recorded at the hackspace

A primer for synthetic biology

Good introduction to gel electrophoresis - Explaining one of the core techniques for working with DNA
Good introduction to the Polymerase Chain Reaction (PCR) - Explaining one of the core techniques for working with DNA
Nature.com article on Biohacking - Free access. Nice explanation of what's needed in a lab and how different groups are managing it.
Bioinformatics Resources
Slides from Bioinformatics workshop August 2012
Cathal Garvey's beginner's guide to biotech
Flowchart diagram of DNA extraction protocol

Community

Interesting DIYBio community blog - Interesting blog taking articles from a few different groups
Bio Curious - A well-established biohacking group in California
Brain-Computer interface at Paris hackspace
Nature.com article about biohacking community - Not the same one as above. Interesting, but behind a paywall :(.
- you can access it here: http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf -- kanzure 70.114.205.110 19:40, 2 January 2011 (UTC)

Proposed code of conduct

See here: http://wiki.hackspace.org.uk/wiki/Biohacking/Code_of_Conduct

Retrieved from ‘https://wiki.london.hackspace.org.uk/w/index.php?title=Group:Biohacking&oldid=52868’

Categories:

@@ Line 1: / Line 1: @@
-We are the '''biohacking''' or '''DIY biology''' ([http://openwetware.org/wiki/DIYbio/FAQ Official FAQ about DIY-Biology]) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved! It's fascinating, the field and community is growing all the time, together with the ability of amateurs to do cool stuff. Over the past year we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short [http://www.bbc.co.uk/news/uk-england-london-19964886 local news video] showing our lab and some of what we do in it.
+{{Group
+|logo=Biohack_logo.png
+|name=Biohacking
+|when=Every Wednesday
+|what=
+|time=19:30
+|recurring=1}}
+We are the '''biohacking''' or '''DIY biology''' ([http://openwetware.org/wiki/DIYbio/FAQ Official FAQ about DIY-Biology]) group at the London Hackspace, a mix of amateur and professional biologists, attracted by the potential of molecular and synthetic biology. Anyone is welcome, so get involved!
+Over the past couple of years we've been developing our equipment, optimising techniques and running some research projects. Here's quite a good short [http://www.bbc.co.uk/news/uk-england-london-19964886 local news video] showing our (previous, smaller) lab and some of what we do in it.
+We will soon be meeting every month, check back here for our 2019 restart.
+We have a list of [[Biohacking FAQ|frequently-asked questions]].
 [[:Category:Biohacking|'''List of biohacker wiki pages.''']]
@@ Line 6: / Line 19: @@
 == How to find us ==
-# Come to the [[Weekly_Open_Evenings|Hackspace Open Evening]] - every tuesday from 7:30pm. There should be a few of us around to show you the lab and give you an idea of what we do. If you want to make sure we find each other you can email us to let us know you're coming.
+# Email us [mailto:hello@biohackspace.org here]. You can also sign up to our mailing list where we do most of our communication [https://lists.kentgeek.org/mailman/listinfo/lhs-biohacking here] (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the [https://groups.google.com/forum/?fromgroups#!forum/london-biohackers google groups] interface. To chat in more detail request a signup to our slack channel using the email address above, or join our irc channel at [http://webchat.freenode.net/?channels=london-biohackers #london-biohackers on freenode]
-# Email us [mailto:hello@biohackspace.org here]. You can also sign up to our mailing list where we do most of our communication [https://lists.kentgeek.org/mailman/listinfo/lhs-biohacking here] (if you see a warning ignore it) (Your posts will be moderated unless you're signed up to the list. If you want to be signed up to the list but not receive messages you can change this in the options). You can view the mailing list archives through the previous link or through the [https://groups.google.com/forum/?fromgroups#!forum/london-biohackers google groups] interface.
+== Biohackspace Membership ==
+We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Therefore it's London Biohackers also need to be members of the hackspace. You can find out how to become a hackspace member [https://london.hackspace.org.uk/signup.php here].
+There is a separate subscription for the biohacking group. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay.  Details on how to setup payment to become a biohackspace member are here: [[London Biohackers Membership | Details of Membership]].
+We also have an arrangement for people who just want to support the biohackspace work and lab costs, and do not intend to regularly attend the biolab to do science. If you want to become a remote member, or to donate equipment and make a regular contribution, and you can make contact [mailto:hello@biohackspace.org here by email], or see the [[Remote Membership|Donations/Remote members]] page for more details.
+However it's not essential to become a member just to come along initially and find out what we do.
-== Membership ==
+== Organization ==
-We're a subgroup of the London Hackspace, and our rent and utility bills are paid as part of the larger space. Most of us are paying members of the hackspace, you can find out how to become a hackspace member [https://london.hackspace.org.uk/signup.php here]. However it's not essential to become a member just to come along initially and find out what we do.
-We also have a special membership for biohacking. Biohacking is more expensive than the typical hackspace activity, and with your membership we can pay for reagents, lab consumables and any equipment we may need for our experiments. The suggested minimum donation is £2 a week, but again, you can come along without joining. If you start to come regularly and use consumables then we'd like you to pay. To find out how to pay biohacking membership, [mailto:membership@biohackspace.org contact us]
+The biohackspace operates as a Company Limited by guarantee. The legal details of this are [[London Biological Laboratories|here]]
 == Projects ==
-We're currently working on getting competent in various techniques of synthetic biology: DNA extraction, PCR, gel electrophoresis, ligation and transformation. Once we can do this, and have been certified as a class 1 lab, we plan to start synbio experiments.
+We are currently well equipped to carry out molecular biology experiments.  The lab space on Hackney Road was a CL1 approved lab and we are aiming to reestablish that in Wembley.
 === Current Projects ===
+* Hacking the lab
+* * Recommissioning RO water system
+* * Recommissioning Laminar Flow system
+* * Building Laminar Flow System into a hood
+* Culturing Local Algae
+* Lab Automation
+* Stirling Engine for Cryogenics
+=== Completed Projects ===
+* [http://2015.igem.org/Team:London_Biohackspace iGEM 2015]
 * [[Project:iGEM 2014]]
 * [[Project:Flow Hood]]
-* [[E. coli GFP transformation]]
+* [[E. coli transformation]]
+* [[Image Focus Stacking]]
-=== Completed Projects ===
+* [[CL1_BSO_SOP_GLP|Lab Containment Level 1]]
 * [[Project:Growing bacteria|Growing V. fischeri]]
 * [[Project:Sex typing with amelogenin]]
@@ Line 29: / Line 60: @@
 * [[Project:Plant species testing]]
 * [[Project:Gel electrophoresis box]]
+* [[Project:Shaker platform]]
 === Future Projects ===
-* [[Project:Algal biodiesel]]
 * [[Project:Making our own reagents]]
-* [[Project:Alcohol Flush Genotyping]]
+*[[MindControlBacteria]]
-* [[Project:BioActivator]]
+* More and more becoming current project :)
-* [[Project:Biohackers microscope]]
+Add your own!
 == Our lab ==
 Our lab is small in size but we have tried to make good use of the tight space to incorporate all the equipment we need to carry out interesting projects. You can see a [[Lab|floor plan of the lab here]].
-We are currently working towards being certified as a Containment Level 1 Lab ([[BioSafety|more details on biosafety classifications]]).
+We <strike> are currently working towards being </strike> are a certified Containment Level 1 Lab ([[BioSafety|more details on biosafety classifications]]).
 The Lab is the registered office of our limited company, [[London Biological Laboratories]], used to deal with suppliers.
@@ Line 48: / Line 79: @@
 Our [[BioLab Manual|lab manual]] details our equipment, reagents, strains and our established protocols.
+=== Starting your own lab? ===
+We have some notes on [[Starting your own biolab|starting your own biolab]] based on our experiences.
 === Equipment we made ===
@@ Line 54: / Line 88: @@
 * [[Project:Gel electrophoresis box]]
 * [[Biobot]]
+* [[Project:Shaker platform]]
+A list of biohacking hardware projects is here [[Project:Biohardware]]
 === Equipment Pledges ===
@@ Line 61: / Line 98: @@
 * [[Pledge: Flow Hood]]
 * [[Pledge:Lab Microscope]]
+* [[Pledge:vacum forming machine]]
+* [[Pledge:Gluconacetobacter_hansenii]]
 == Resources ==
@@ Line 79: / Line 118: @@
 === Community ===
 *[http://diybio.org/blog Interesting DIYBio community blog] - Interesting blog taking articles from a few different groups
-*[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established bihacking group in California
+*[http://biocurious.org/index.php?title=Main_Page Bio Curious] - A well-established biohacking group in California
 *[http://www.tmplab.org/wiki/index.php/Brain_Computer_Interface Brain-Computer interface at Paris hackspace]
 *[http://www.nature.com/nbt/journal/v27/n12/full/nbt1209-1077.html Nature.com article about biohacking community] - Not the same one as above. Interesting, but behind a paywall :(.
 ** you can access it here: [http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf http://www.synbioproject.org/process/assets/files/6452/_draft/nbt-2009-12d_-_biotech_nin_the_basement.pdf] -- [http://heybryan.org/ kanzure] [[Special:Contributions/70.114.205.110|70.114.205.110]] 19:40, 2 January 2011 (UTC)
-=== PCR primer design ===
-* http://www.cybertory.org/exercises/primerDesign/index.html
-* http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html
-* http://www.premierbiosoft.com/primerdesign/index.htm
-* http://molbiol-tools.ca/PCR.htm
-* http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome
-* http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi
-* http://bibiserv.techfak.uni-bielefeld.de/genefisher2/
 == Proposed code of conduct ==